Four new steroidal glycosides from solanum torvum and their cytotoxic activities. Jan 01, 2009 read four new steroidal glycosides from solanum torvum and their cytotoxic activities, steroids on deepdyve, the largest online rental service for scholarly research with thousands of academic publications available at your fingertips. Their structures were established by 2d nmr spectroscopic techniques 1 h, 1 hcosy, tocsy, noesy, hsqc, and hmbc and mass spectrometry as 25s26. Using bioactivitydirected isolation procedures, five new spirostan saponins and two sterol glycosides have been isolated from solanum chrysotrichum leaves. Two new steroidal glycosides isolated from the aerial part. There is not much research on chemical composition of solanum torvum roots, but steroidal glycosides astorvosides ag and jurubine have been isolated.
Antiviral isoflavonoid sulfate and steroidal glycosides from the fruits of solanum torvum. Antiviral isoflavonoid sulfate and steroidal glycosides were also isolated from the fruits of s. Torvpregnanosides a and b, two pregnane glycosides, and torvoside q, a 23ketospirostanol glycoside, along with twelve known steroidal saponins were isolated from aerial parts of solanum torvum. While the mass spectra of the compounds found in the extract was matched by the national institute of standards and technology nist library. Conclusion the extensive literature survey revealed that solanum torvum is an important source of many pharmacologically and medicinally important chemicals such as steroidal glycosides, sitosterol, stigmasterol and campesterol. Solasodine accumulation in regenerated plants of solanum torvum sw. The free radical scavenging effects of solanum torvum on dpph 2, 2diphenyl1picrylhydrazyl was measured invitro. Three chromatographically individual fractions, each containing tigogenin glycosides and diosgenin glycosides have been isolated by chromatography on a silica gel column from a methanolic extract of eggplant seeds. Five new steroidal saponins from solanum chrysotrichum. Arthan d1, svasti j, kittakoop p, pittayakhachonwut d, tanticharoen m, thebtaranonth y.
Solanaceae is traditionally used for management of various ailments. Thus, in this paper, we report the isolation and identification of two steroidal alkaloid glycosides 1 and 2 and chlorogenic acid 3 from the fruits along with steroidal glycosides from aerial parts and tubers fig. Previously, we reported seven steroidal glycosides from the aerial parts and the roots of this plant. Solanaceae, solanum torvum, alkaloids, solasodine, solamargine.
Two new steroidal glycosides isolated from the aerial part of solanum torvum swartz. Challal s1, buenafe oe, queiroz ef, maljevic s, marcourt l, bock m, kloeti w, dayrit fm, harvey al, lerche. Antimicrobial and antimycobacterial activities of methyl. In the present study, solanum torvum sundakai was selected. Pharmacognostical studies and evaluation of total phenolic. Steroidal saponins could be found in many species of the solanum, and they are an important group of natural products exhbiting a number of potent beneficent properties, such as antitumor, antibacterial, antifungal, antiinflammatory, antiviral, antioxidant, hypoglycemic, and antihyperlipidemic effects. Seven steroidal glycosides have been isolated from the fruits of solanum torvum swartz. The main aim of this study was to find a new natural, non toxic, effective antibiotic from plant extract.
In contrast with the potato aphid macrosiphum euphorbiae, s. The chemical compositions of methanol extract leaves of solanum torvum were investigated using perkinelmer gas chromatographymass spectrometry. Solasonine also occurs in various solanum species namely. This study determined the phytochemical constituents and antibacterial activity of the ethanolic extract of s. Protective effect of solanum torvum on monosodium glutamateinduced neurotoxicity in mice mahalaxmi mohan, sushilkumar gangurde, vrushali kadam. Besides, the berries contain a steroidal glycoalkaloid known as solasodine approx.
Four new steroidal glycosides from solanum torvum and. In addition, solanum torvum exhibited an antioxidant. Protective effect of solanum torvum on doxorubicininduced. Their structures were elucidated on the basis of spectroscopic techniques 1d and 2d nmr, hreims, hrfabms, ir, physical data, and chemical analysis. Antimycotic spirostanol saponins from solanum hispidum. Pdf antiviral isoflavonoid sulfate and steroidal glycosides. Fruit fruit chandrasekhar balachandran, 1 veeramuthu duraipandiyan, 1, 2 naif abdullah aldhabi, 2 k.
Cytotoxic activity of steroidal glycosides from solanum. Traditional medicinal uses solanum torvum is a pharmacologically important species of the family solanaceae. Six new steroidal saponins, solanigrosides ch, and one known saponin. Phytochemical screening and gcms, ftir analysis of.
Interestingly, these group of alkaloids invariably occur in the plant kingdom as glycosidal combination with carbohydrate moieties. Spirostanol saponins, 16 and 1819 normaltype f ring, isolated from solanum torvum and s. Solanum chacoense solanaceae chaco potato developmental pro. Pharmacognostical studies and evaluation of total phenolic and flavonoid contents of traditionally utilized fruits of solanum torvum sw. An antineutrophilic inflammatory investigation for solanum torvum was conducted. All structures were identified from spectroscopic data, and the compounds were tested for antineutrophilic inflammation activity. Antineutrophilic inflammatory steroidal glycosides from. Phytochemistry and pharmacological studies on solanum. Keywords solanum torvum swartz, steroidal glycoside, solanolactoside c, torvoside q. Solanacea is a popularly used medicine in tamilnadu for treating various illnesses in the native system of. The roots are reported to act as antibacterial, antiviral, antifungal, antiinflammatory and antitumor. This finding correlates well with the ethnomedical use of this plant in the philippines, where a water decoction of s.
In addition to khasianine, solamargine, xylosylsolamargine and solasonine, three steroidal alkaloid glycosides, solasuaveoline, dihydrosolasuaveoline and isosolasuaveoline, have been isolated from aerial parts of solanum suaveolens. Steroidal glycosides from the fruits, aerial parts. Looking for online definition of steroidal solanum spp. Antiviral isoflavonoid sulfate and steroidal glycosides from. Two novel c22 steroidal lactone saponins, namely solanolactosides a, b and two new spirostanol glycosides, namely torvosides m, n were isolated from ethanol extract of aerial parts of solanum torvum and their structures were characterized on the basis of spectroscopic analysis 7. Solanum torvum is a bushy, erect and spiny perennial plant used horticulturally as a rootstock for eggplant.
Four new steroidal glycosides from solanum torvum and their. Solanum torvumswartz solanaceae is a small shrub, of which fruits are commonly available in the markets and are used as a vegetable regarded as an essential ingredient in thai cuisine. The structure of these compounds was established based upon spectroscopic measurements, especially 1d and 2d nmr data of their peracetate derivatives. Steroidal saponins and their pharmacological activities. Read four new steroidal glycosides from solanum torvum and their cytotoxic activities, steroids on deepdyve, the largest online rental service for scholarly research with thousands of academic publications available at your fingertips.
The objective of this research was to screen potential cytotoxicity of these potato glycoalkaloids using bovine. Torvoside al 8, 9,10, among these torvoside a, torvoside b and torvoside e, torvoside f, torvoside g, torvoside h, are considered to be furostanol glycosides 11,12. Petroleum ether, chloroform, ethyl acetate and methanol extract of leaves in solanum torvum. In this time, three unusual 22bospirostanol glycosides have been isolated from the fruits of this plant. Different parts of the plant are reported to possess a number of medicinal properties. Their chemical structures were determined by analysis of hresims and nmr spectra. Solanum torvum contains a number steroidal glycosides viz. Some researchers found the presence of ascorbic acid in the fruits of solanum nigrum and the concentration of ascorbic acid is more in fruit than root 21. The structures of 1 and 2 were determined by extensive nmr experiments including 1h nmr, c nmr, dept, 1h1h cosy, hsqc, hmbc and roesy and hresims to be solanolide 6o. The hydroalcoholic extract of fruits of this plant at dose level 200 mg kg b w showed promising hepatoprotective activity as evidenced by. Aaaaccccaaaaddddeeeemmmmiiiicccc ssssccciiieeeennnnccceeeesss.
Common solanum glycosides were tested against a non solanum pest, the greenbug, schizaphis graminum, fed by artificial diet supplemented with the tested compounds. Four new steroidal saponins, anguiviosides iii 1, xi 2, xv 3, and xvi 4, were isolated and characterized from the fruits of solanum anguivi. Alkaloids in solanum torvum sw solanaceae phyton international. Zebrafish bioassayguided microfractionation identifies. Five new steroidal glycosides together with five known ones were isolated from the fruits of s. Queiroz 1, snezana maljevic 3, laurence marcourt 1, werner kloeti 1, fabian m. The fruits of solanum torvum swartz, commonly known as turkey berry, are edible and commonly used as a vegetable in the south indian populations diet and as an essential ingredient in thai cuisine. To isolate a flavonoid glycoside from flowers of solanum torvum using chromatography separation techniques, spectral characterization and its antidiabetic activity.
Steroidal glycosides from solanum torvum sciencedirect. Protective effect of solanum torvum on monosodium glutamate. Two new steroidal saponins, solaprocumosides a 1 and b 2, and a known compound paniculonin b 3 were isolated from the aerial parts of solanum procumbens. Balakrishna, 1 nitin pal kalia, 3 vikrant singh rajput, 3 inshad ali khan, 3 and savarimuthu ignacimuthu 1. Two new steroidal saponins from solanum procumbens truong. Recently, an additional steroidal glycoside, torvoside h8 was reported. Two new steroidal saponins from solanum procumbens. In general, the steroidal alkaloids represent an important class of alkaloids that essentially afford a close structural relationship to sterols i. Queiroz1, snezana maljevic3, laurence marcourt1, werner kloeti1, fabian m. Two new steroidal glycosides from solanum surattense.
Effects of steroidal glycoalkaloids from potatoes solanum. The compounds were isolated by various chromatographic techniques and their structures were elucidated on the basis of extensive spectroscopic analysis, chemical methods, and comparison with reported spectroscopic data. Two new steroidal alkaloid glycosides, solanoside a 1 and solanoside b 2, were isolated from the whole plant of solanum surattense. Rats were randomly divided into 5 groups, each of n 6.
Five new cyotoxic steroidal glycosides from the fruits of. Of the latter, four of the 23hydroxyspirostanol glycosides, and, a yamogenin glycoside. Two novel c22 steroidal lactone saponins, namely solanolactosides a, b and two new spirostanol glycosides, namely torvosides m, n were isolated from ethanol extract of aerial parts of solanum torvum and their. Philippine herbal medicine an illustrated compilation of philippine medicine plants by dr godofredo stuart, with botanical information, chemical properties, folkloric uses and medicinal research studies. Diuretic and serum electrolyte regulation potential of.
Their structures were elucidated on the basis of spectroscopic analysis. Two new steroidal glycosides isolated from the aerial part of. The c4 sulfated isoflavonoid, torvanol a 1, and the steroidal glycoside, torvoside h 3, together with the known glycoside, torvoside a 2, were isolated from a meoh extract of solanum torvum fruits. Solanine and chaconine are two naturally occurring steroidal glycoalkaloids in potatoes solanum tuberosum, and solanidinenoxide is a corresponding steroidal aglycone.
Steroidal saponins from the fruits of solanum torvum. The antibacterial activity of different extracts of solanum torvum sundakai fruit coat was studied in vitro against human pathogenic strains. Pdf phytochemistry and pharmacological studies on solanum. The present study aimed at determining the chemical constituents of solanum coagulans and their antimicrobial activities. The result revealed that the methanolic extract of solanum torvum recorded the presence of alkaloid, flavonoid, phenol, tannins, saponins, phytosterol and glycosides followed by other extract table. Their structures were elucidated on the basis of spectroscopic techniques 1d and 2d nmr, hreims, hrfab. Steroidal saponins from solanum procumbens growing in vietnam.
Reports on isolation of antiviral isoflavonoid sulfate and steroidal glycosides from fruits were also confirmed as per arthan et al. Muhammad arif and sheeba fareed faculty of pharmacy, integral university, kursi road, lucknow260226, uttar pradesh, india received 18 june 2010. Effect of solanum glycosides on the aphid schizaphis graminum. Torvonina, solasonine, sterolin sitesteroldglucoside, protein, fat and minerals have been. Chemical constituents of solanum coagulans and their. Two novel c22 steroidal lactone saponins, namely solanolactosides a, b and two new spirostanol glycosides, namely torvosides m, n were. The study was conducted for provision of pharmacological justification for folkloric uses of solanum surattense in the treatment of dysuria. Antiviral isoflavonoid sulfate and steroidal glycosides. Three unusual 22b 23hydroxy5aspirostanol glycosides. Since some solanum genera plants have traditionally been used as anticancer and antiherpes agents from olden times, we examined the cytotoxic activity of typical steroidal glycosides with the. Arthan d, svasti j, kittakoop p, pittayakhachonwut d, tanticharoen m, thebtaranonth y 2002 antiviral isoflavonoid sulfate and steroidal glycosides from the fruits of solanum torvum. Steroidal alkaloid glycosides from solanum suaveolens. Antineutrophilic inflammatory steroidal glycosides from solanum.
Challal, soura and buenafe, olivia erin m and queiroz. Antibacterial activity of different extracts of sundakai. Three steroidal glycosides were isolated and characterized from s. Pdf chemical constituents from solanum torvum researchgate. Isolation, identification and antidiabetic activity of. Zebrafish bioassayguided microfractionation identifies anticonvulsant steroid glycosides from the philippine medicinal plant solanum torvum by soura challal, olivia erin m buenafe, emerson ferreira queiroz, snezana maljevic, laurence marcourt, merle bock, werner kloeti, fabian millar dayrit, alan l harvey, holger lerche, camila v esguerra. Medicinal plants used for the treatment of epilepsy are potentially a valuable source of novel antiepileptic small molecules.
New steroidal glycosides from the fruits of solanum. The etoacsoluble fractions of solanum torvum and s. Seven new steroidal glycosides, named torvosides ag, were isolated from the aerial parts and from the roots of solanum torvum. In this chapter we describe the use of uplc ultra performance liquid chromatography coupled with qtof quadrupole timeofflight mass spectrometry for profiling of these two large classes of semipolar. Solanacea is a popularly used medicine in tamilnadu for treating various illnesses in the native system of medicine. Solanum torvum, solanum macaonense, solanaceae, steroidal glycoside, cytotoxicity, antiinflammation.
Phytochemical analysis and antibacterial activity of. Five new steroidal saponins from solanum chrysotrichum leaves. Alteration in antioxidantactivity and phytochemical compound. Zebrafishbioassay guided microfractionation identifies. The objective of the study was to evaluate the effects of s. Antimicrobial and antimycobacterial activities of methyl caffeate isolated from solanum torvum swartz. The structures of 1 and 2 were determined by extensive nmr experiments including 1h nmr, c nmr, dept, 1h1h cosy, hsqc, hmbc and roesy and hresims to be. Torvpregnanosides a and b 12, two pregnane glycosides, and torvoside q 3, a 23ketospirostanol glycoside, along with twelve known steroidal saponins were isolated from solanum torvum. The occurrence of the cholestane glycosides 3 and 4 is considered from a biogenetic point of view. The c4 sulfated isoflavonoid, torvanol a 1, and the steroidal glycoside, torvoside h 3, together with the known glycoside, torvoside a 2, were isolated from a meoh extract of solanum torvum. To identify anticonvulsant secondary metabolites, we performed an in vivo, zebrafishbased screen of medicinal plants used in southeast asia for the treatment of seizures.
Solanum torvum is a well known traditional herbal medicinal plant used in many neurological disorders. Secondary metabolites from the fruits of solanum torvum sw. Phytochemistry and pharmacological studies on solanum torvum. One novel c22 steroidal lactone saponin, namely solanolactoside c 1, and one new spirostanol glycoside, namely torvoside q 2, were isolated from the ethanolic extract of aerial parts of solanum torvum swartz. To separate the mixture of two difficultly separable glycosides into individual components, each fraction was acetylated and epoxidated, and the derivatives obtained were. The structure of the isolated compound was purified, analyzed and characterized by chemical tests, hplc and spectroscopic methods such as uv, ir, 1hnmr and cnmr. One novel c22 steroidal lactone saponin, namely solanolactoside c 1, and one new spirostanol. Compound 1 had ketone functional groups at c16 and c22 of sterol skeleton which is rarely found in nature. Plants of the solanaceae family are renowned for the production of cholesterolderived steroidal glycosides, including the nitrogen containing glycoalkaloids and steroidal saponins. Solanaceae was identified as having significant anticonvulsant activity in zebrafish larvae with seizures induced by the gaba a antagonist pentylenetetrazol ptz. Zebrafish bioassayguided microfractionation identifies anticonvulsant steroid glycosides from the philippine medicinal plant solanum torvum. Solanaceae was identified as having significant anticonvulsant activity in. New steroidal glycosides from the fruits of solanum anguivi.
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